Esterification of glycerol 3-phosphate in lactating guinea-pig mammary gland.
نویسنده
چکیده
1. The presence of palmitoyl-CoA-l-glycerol 3-phosphate palmitoyltransferase (EC 2.3.1.15) has been demonstrated in a particulate fraction of mammary tissue from lactating guinea pigs. 2. Cell-free preparations also catalysed the activation of palmitate and oleate, and the conversion of enzymically formed phosphatidic acid into glycerides, in accord with the Kennedy pathway of glyceride formation. 3. The properties of the system that esterifies l-glycerol 3-phosphate were studied with respect to substrates and cofactors, and the reaction product was shown to be phosphatidic acid (1,2-diacyl glycerol 3-phosphate). 4. The extent to which newly formed phosphatidic acid was converted into glyceride in a cell-free system was dependent on the nature of the acyl donor, the concentration of subcellular particles, the time of incubation and the concentration of Mg(2+).
منابع مشابه
Acceptors of fatty acid for glyceride synthesis in guinea pig mammary gland.
T w o PATHWAYS have now been described for the biosynthesis of glycerides in mammalian tissues. In the pathway which exists in liver (1, 2) and adipose tissue (3), triglycerides are synthesized from L-a-glycerophosphate via L-a-phosphatidic acids and D-a,@-diglycerides. More recently it has been found that a second pathway involving the direct acylation of monoglyceride exists in intestinal muc...
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ورودعنوان ژورنال:
- The Biochemical journal
دوره 105 1 شماره
صفحات -
تاریخ انتشار 1967